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Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes
https://cis.repo.nii.ac.jp/records/269
https://cis.repo.nii.ac.jp/records/269097cf630-90a0-4ec5-a54b-893fbdffa605
| 名前 / ファイル | ライセンス | アクション |
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001-010 足立達美先生.pdf (6.7 MB)
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copyright (c) 2018 by Chiba Institute of Science
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2019-06-17 | |||||
| タイトル | ||||||
| タイトル | Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes | |||||
| タイトル | ||||||
| タイトル | Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
足立, 達美
× 足立, 達美× 片本, 祐輔× 岩館, 誠美× 柳沢, 泰任× 安田, 一郎× Katamoto, Yusuke× Iwatate, Tomomi× Yanagisawa, Yasuhide× Yasuda, Ichiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Region-dependent differences in morphological changes induced by an increment of cAMP, and an exposure with either mercury compounds or lysophosphatidic acid (LPA), a Rho activator, were examined in cultured astrocytes from the cerebral hemisphere and cerebellum of newborn rats. When the cells were maintained in a serum-containing medium, the morphology was a flat polygonal shape, and prominent actin stress fibers were observed in astrocytes from both regions. After changing the medium to a serum-free one containing 0.5 mM dibutyryl cyclic AMP (dbcAMP), the morphology changed to a process-bearing stellate shape together with a loss of stress fibers in both astrocytes. When methylmercury (MeHg) was exposed to the cells at 3 μM for 3 h immediately after the medium change, most cerebral hemisphere astrocytes showed a polygonal shape, together with the formation of stress fibers, whereas only a few cerebellar astrocytes did so. In contrast, inorganic mercury did not influence the morphology in either astrocyte at that concentration. Moreover, similar to MeHg, LPA also region-dependently induced spreading of astrocytes, suggesting that Rho proteins might be key molecules in the MeHg-induced alteration in astrocyte morphology. The present results suggest that morphologies in the presence of serum and dbcAMP-stimulated stellation are similar in the cerebral hemisphere and in cerebellar astrocytes in cultures, and that there are marked region-dependent differences in responses to LPA and mercury compounds, especially MeHg-induced shape changes (spreading) and actin reorganization. | |||||
| 書誌情報 |
千葉科学大学紀要 en : The Universal Bulletin of Chiba Institute of Science 号 12, p. 1-10, 発行日 2019 |
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| 出版者 | ||||||
| 出版者 | 千葉科学大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1882-3505 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA1230240X | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
