{"created":"2023-06-19T07:46:26.407159+00:00","id":269,"links":{},"metadata":{"_buckets":{"deposit":"7d1c130a-a463-41a3-b139-aa31698e94ff"},"_deposit":{"created_by":15,"id":"269","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"269"},"status":"published"},"_oai":{"id":"oai:cis.repo.nii.ac.jp:00000269","sets":["3:25"]},"author_link":["950","949","953","951","948","954","955","952","947"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"千葉科学大学紀要"},{"bibliographic_title":"The Universal Bulletin of Chiba Institute of Science","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Region-dependent differences in morphological changes induced by an increment of cAMP, and an exposure with either mercury compounds or lysophosphatidic acid (LPA), a Rho activator, were examined in cultured astrocytes from the cerebral hemisphere and cerebellum of newborn rats. When the cells were maintained in a serum-containing medium, the morphology was a flat polygonal shape, and prominent actin stress fibers were observed in astrocytes from both regions. After changing the medium to a serum-free one containing 0.5 mM dibutyryl cyclic AMP (dbcAMP), the morphology changed to a process-bearing stellate shape together with a loss of stress fibers in both astrocytes. When methylmercury (MeHg) was exposed to the cells at 3 μM for 3 h immediately after the medium change, most cerebral hemisphere astrocytes showed a polygonal shape, together with the formation of stress fibers, whereas only a few cerebellar astrocytes did so. In contrast, inorganic mercury did not influence the morphology in either astrocyte at that concentration. Moreover, similar to MeHg, LPA also region-dependently induced spreading of astrocytes, suggesting that Rho proteins might be key molecules in the MeHg-induced alteration in astrocyte morphology. The present results suggest that morphologies in the presence of serum and dbcAMP-stimulated stellation are similar in the cerebral hemisphere and in cerebellar astrocytes in cultures, and that there are marked region-dependent differences in responses to LPA and mercury compounds, especially MeHg-induced shape changes (spreading) and actin reorganization.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"千葉科学大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1230240X ","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1882-3505","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"足立, 達美"},{"creatorName":"アダチ, タツミ","creatorNameLang":"ja-Kana"},{"creatorName":"Adachi, Tatsumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"947","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005261460","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005261460"}]},{"creatorNames":[{"creatorName":"片本, 祐輔"},{"creatorName":"カタモト, ユウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"948","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩館, 誠美"},{"creatorName":"イワタテ, トモミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"949","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柳沢, 泰任"},{"creatorName":"ヤナギサワ, ヤスヒデ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"950","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000000327792","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000327792"}]},{"creatorNames":[{"creatorName":"安田, 一郎"},{"creatorName":"ヤスダ, イチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"951","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003172999","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003172999"}]},{"creatorNames":[{"creatorName":"Katamoto, Yusuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"952","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwatate, Tomomi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"953","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagisawa, Yasuhide","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"954","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"955","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-06-17"}],"displaytype":"detail","filename":"001-010 足立達美先生.pdf","filesize":[{"value":"6.7 MB"}],"format":"application/pdf","license_note":"copyright (c) 2018 by Chiba Institute of Science","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"001-010 足立達美先生.pdf","url":"https://cis.repo.nii.ac.jp/record/269/files/001-010 足立達美先生.pdf"},"version_id":"6c6ef77b-aa9d-41f1-ba32-df20c4227805"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes"},{"subitem_title":"Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"15","path":["25"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-06-17"},"publish_date":"2019-06-17","publish_status":"0","recid":"269","relation_version_is_last":true,"title":["Region-Dependent Differences in Morphological Changes Induced by Mercury Compounds and Lysophosphatidic Acid in Cultured Rat Astrocytes"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-19T07:53:36.351700+00:00"}