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Suppression of Senescent Phenotypes in Normal Human Diploid Cells by Nicotinamide and phosphoinositide 3-kinase inhibitors
https://cis.repo.nii.ac.jp/records/338
https://cis.repo.nii.ac.jp/records/3388f7b4cbf-e549-4a91-b5d4-d54743966ecd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6-14松岡耕二 (1.3 MB)
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copyright (c) 2020 by chiba Institute of Science
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2021-04-05 | |||||
| タイトル | ||||||
| タイトル | Suppression of Senescent Phenotypes in Normal Human Diploid Cells by Nicotinamide and phosphoinositide 3-kinase inhibitors | |||||
| タイトル | ||||||
| タイトル | Suppression of Senescent Phenotypes in Normal Human Diploid Cells by Nicotinamide and phosphoinositide 3-kinase inhibitors | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
松岡, 耕二
× 松岡, 耕二× 佐々木, 啓子× CHEN, Kuang Yu× MATUOKA, Koozi× SASAKI, Keiko× CHEN, Kuang Yu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Normal human diploid cells undergo physiological aging by serial subcult ure in vitro as defined by the loss of doubling potential, the appearance of senescence associated β galactosidase activity, a decrease of cellular motility and a change of cell morphology . Alternatively, these phenotypes can be induced by exposing young cells to a brief oxidative stress such as hydrogen peroxide treatment. We have previously reported that nicotinamide (NAM), a vitamin and energy metabolism related molecule, reversibly suppresses the senescent phenotypes associated with physiological aging as well as the stress induced aging. The aim of the present study is to inves tigate whether other small molecules that are known to be involved in modulating key signaling pathways may act like NAM in affecting the senescent phenotypes. Specifically, we found the activators for sirtuin, AMP kinase and autophagy signaling all suppress the senescent phenotypes, whereas their inhibitors induce senescent phenotypes. On the other hand, the inhibitors of phosphoi nositide 3 kinase (PI3K) suppressed senescent phenotypes. To further analyze the role of NAM and PI3K in the senescence modulati ng process, we examined the expression of various PI3K catalytic units and the phosphorylation of Akt, a PI3K downstream target. Our study suggested that the expression of senescent phenotypes in human diploid cells is intimately connected to signaling ne tworks involved in energy metabolism and autophagy. |
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| 書誌情報 |
千葉科学大学紀要 en : The University Bulletin of Chiba Insitute of Science 号 14, p. 6-14, 発行日 2021-03-28 |
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| 出版者 | ||||||
| 出版者 | 千葉科学大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2436-2565 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
