@article{oai:cis.repo.nii.ac.jp:00000306,
 author = {SASAKI, Keiko and WADA, Keiji and Kobayashi, Daisuke and MURAKAMI, Akira and MATUOKA, Koozi and SASAKI, Keiko and WADA, Keiji and Kobayashi, Daisuke and MURAKAMI, Akira and MATUOKA, Koozi},
 issue = {13},
 journal = {千葉科学大学紀要, The University Bulletin of Chiba Institute of Science},
 month = {Feb},
 note = {In order to characterize the biological actions of zerumbone (ZER), a cancer-suppressive component
from rhizomes of Zingiber zerumbet Smith, its effects on the activities of various xenobiotic-metabolizing
enzymes were analyzed. Mice were treated orally with ZER (10-100 mg/kg, once a day for 4 successive
days) and then the enzyme activities in mouse organs were examined. Within the liver ZER increased
glutathione S-transferase (GST) and quinone reductase (QR) activities in a dose-dependent manner up to
204% and 168% of the control, respectively. As the GST activation was most apparent with 1,2-dichloro-4-
nitrobenzene as substrate, ZER seems to mainly induce the activity of class μ GST, which was supported by
the result of a Western blotting experiment. In addition, contents of glutathione (reduced form) were slightly
higher in the ZER-treated liver. Similarly, small intestine GST and QR activities elevated to 861% and 256%
of the control, respectively, upon ZER treatment. In contrast, the activities of those enzymes fl uctuated little
in kidney, lung or brain of ZER-treated mice. As for the phase I enzymes in the liver including cytochrome
P450 and cytochrome b5; activities of 7-ethoxycoumarin O-deethylase, 7-methoxyresorufi n O-demethylase,
7-ethoxyresorufi n O-deethylase and 7-pentoxyresorufi n O-depentylase , ZER caused only marginal changes.
Our study clearly indicated that ZER selectively activates phase II enzymes in the liver and small intestine, and
thus can be considered to be a promising nutraceutical for cancer chemoprevention.},
 pages = {11--20},
 title = {Zerumbone Elevates Phase II, but not Phase I, Xenobiotic-Metabolizing Enzymes in Mouse Liver},
 year = {2020}
}