{"created":"2025-03-21T07:13:10.248175+00:00","id":2000099,"links":{},"metadata":{"_buckets":{"deposit":"69856980-648d-4952-b252-0f24a61d928b"},"_deposit":{"created_by":15,"id":"2000099","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000099"},"status":"published"},"_oai":{"id":"oai:cis.repo.nii.ac.jp:02000099","sets":["3:1741764459278"]},"author_link":["118"],"item_10002_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-03-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicNumberOfPages":"4","bibliographicPageEnd":"66","bibliographicPageStart":"63","bibliographic_titles":[{"bibliographic_title":"千葉科学大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"The University Bulletin of Chiba Institute of Science","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"細胞診標本の固定には通常95％エタノールが用いられるが、この固定液が細胞中の抗原の固定に最適かどうかは多く検討されてこなかったこともあり、最近ではホルマリンで固定した検体よりスクラッチして細胞を採取し、LBCの手法で作製すると良好な染色性の標本となることも報告されている。今回はそもそも細胞診の固定液としてどれが最適なのかを検討した。検討材料にはヒト末梢血検体およびヒト尿検体3例ずつを用いた。まず湿固定、乾燥固定の標本を作製し、染色の態度より各染色を0～3まで4段階にスコアリングして評価を行った。パパニコロウ染色、PAS（Periodic acid-Schiff）反応 、免疫染色を行って染色性を検討した。免疫染色についてはLCA、MPO、サイトケラチン、ビメンチンを検討した。免疫染色の検討に際し、抗原賦活化処理は行わなかった。検討した固定液は95％エタノール、メタノール、Liquid Based Cytology（LBC）固定液（TACAS RUBY）、10％緩衝ホルマリン、ホルマリン/酢酸/エタノール混合液の5種類で行った。検討の結果、パパニコロウ染色では乾燥固定標本は湿固定標本と比較して染色性が低下していた。しかし、PAS反応では乾燥固定した標本でも湿固定標本と同様の染色性であった。免疫染色を含めた総合的な評価において、固定液の中では10％緩衝ホルマリン、95％エタノールが高スコアであり、湿固定の場合、Pap染色や免疫染色で従来行われているエタノール固定よりホルマリン固定で良い染色性を示した。細胞診材料をホルマリン固定することができれば医療現場で固定液を統一できることになり、危機管理上有用である。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_full_name_3":{"attribute_name":"著者（英）","attribute_value_mlt":[{"names":[{"name":"IKEDA, Satoshi","nameLang":"en"},{"name":"FUKUDOME, Nobuyuki","nameLang":"en"},{"name":"IKEGAMI, Kikuo","nameLang":"en"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"千葉科学大学","subitem_publisher_language":"ja"}]},"item_10002_source_id_11":{"attribute_name":"item_10002_source_id_11","attribute_value_mlt":[{"subitem_source_identifier":"AA1230240X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"item_10002_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"2436-2565","subitem_source_identifier_type":"EISSN"}]},"item_10002_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池田, 聡","creatorNameLang":"ja"},{"creatorName":"イケダ, 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